Surface Plasmon Resonance (SPR) Spectroscopy for Signal Enhancement
Surface plasmon resonance (SPR) spectroscopy and localized surface plasmon resonance (LSPR) spectroscopy have been recognized as invaluable tools for label fee chemical and biological sensing; as well as nanostructure characterization. The most common application of SPR spectroscopy is in the field of biosensing, particularly the study of binding affinities, such as antibody-antigen interactions. LSPR spectroscopy, on the other hand, is predominantly used as a signal enhancement technique for trace molecule detection. In this application note, we will discuss the underlying physics behind surface plasmons, including their wavelength-dependent interactions. Next, we will go on to discuss the differences between SPR and LSPR, examining the mechanisms behind signal enhancement. Lastly, we will provide two brief case studies demonstrating applications of both SPR and LSPR spectroscopy.
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