cart My Cart 0
☰ Menu

Fluorescence Spectroscopy

Many organic compounds fluoresce, which is to say they emit light (usually visible but not always) when their electrons are excited by electromagnetic radiation of a lower wavelength. The amount of fluorescence energy emitted is only a small fraction of the energy input, typically only about 3% of excitation, and is more diffuse, scattering light in all directions. These conditions require a powerful spectrometer with high-sensitivity and long integration times to capture fluorescence data.

Fluorescence Spectroscopy, also called fluorometry or spectrofluorometry, is a very powerful tool. However, one important concern when applying this measurement technique is the possibility for stray excitation light to enter the spectrometer. Avantes offers several ways to mitigate this stray excitation light. One might employ a narrow bandwidth AvaLight-LED source to limit excitation wavelengths to those not part of the emission spectrum, or choose an AvaLight-Hal high-output broadband light source along with a bandpass or low-pass filter. A more mechanical solution might be to ensure the light can’t enter the fiber leading into the spectrometer with the use of a cuvette holder (use the CUV-UV/VIS-FL or the CUV-DA) to ensure the excitation path is perpendicular to the optical path for fluorescence measurements. Finally, it is possible to use the rate of fluorescence decay to separate the excitation-emission from the fluorescence measurement using a pulsed light source such as the AvaLight-XE Xenon flash and triggering options onboard the spectrometer.

Avantes offers a basic fluorescence bundle that includes an AvaSpec-ULS2048L high-sensitivity spectrometer along with LED light source and fluorescence probe. For applications that need even higher sensitivity, Avantes offers several options including the AvaSpec-Hero (HSC1024x58Tec-EVO) which perfectly balances high-sensitivity and high-resolution in this TE cooled CCD back-thinned spectrometer.

Avantes also offers a wide selection of fiber optic sampling accessories for fluorescence spectroscopy applications including the cuvette holders, direct attached and in-line filter holders and flow cells to support any fluorescence application need.

Applications for Fluorescence Measurements

There are numerous uses for fluorescence spectroscopy, but one of the largest areas is in the life sciences and biomedical field. Fluorescence spectroscopy is used to diagnose malignancies because cancer cells do not have the same fluorescence response that healthy tissue does, but it is also used to tag genetic markers to facilitate genetic research.

Fluorescence can also be utilized in agricultural applications due to the fluorescent response of chlorophyll and fluorescent dyes can be employed for security measures such as identifying leaking radioactive byproducts in the generation of nuclear electric power.

Read More

Fluorescence Instruments

product image


The StarLine AvaSpec-ULS2048CL-EVO offers you this latest technology using CMOS instead of the conventional CCD technology. In combination with our latest AS-7010 electronics, it offers you a versatile device including USB3.0 communication with 10x higher speed communications and 50x more memory. The AvaSpec-ULS2048CL-EVO is also available as OEM unit, bench-only or rackmount version.

Get Product Information
product image


The AvaSpec-HS2048XL-EVO is an exceptional instrument for high sensitivity applications where high resolution is not of paramount concern. It features the Avantes HS optical bench which has a full 0.22 numerical aperture for superior throughput The AvaSpec-HS2048XL-EVO uses the AS7010 electronics board offering USB3 (10 times faster than USB2), Gigabit Ethernet and better signal processing.

Get Product Information
product image

AvaSpec-HS1024x58-TEC (AvaSpec-Hero)

This spectrometer combines the best of both worlds: high resolution and high sensitivity! The AvaSpec-HERO is build up around our High Sensitivity Compact 100mm optical bench, offering a NA of 0.13 and a cooled Backthinned Detector (1024x58 pixels). The Hero uses the newest AS7010 board, including a High Performance Analog to Digital convertor

Get Product Information

Fluorescence Resources

Fluorescence Information Page

Get Info

Download: Fluorescence Application Note


Fluorescent Measurement Instrument Bundle

Get Info

Video: How to do a Fluorescence Measurement


Fluorescence System Design with Beam Splitter/Combiner

Read More

Fluorescence by Avantes

Design the Ideal Fluorimetry System Today

Start Today

Discover More Recent Applications in Spectroscopy

Posted on: April 23rd, 2024

In-Line Process Optimization with Spectroscopy

Introduction Traditional manufacturing is undergoing a significant transformation due to rapid technological advancements. Modern data science is driving the emergence…

Read More

Posted on: February 27th, 2024

Chemical Identification of Resin with Raman Spectroscopy

Summary Raman spectroscopy is a highly valuable and widely utilized technique for real time chemical identification measurement in various molecules and…

Read More

Posted on: January 25th, 2024

Optimal Spectroscopy Configurations for DOAS

The environmental impact of carbon emissions from fossil fuels, the most widely recognized and well-known air pollutant, is undeniable. However,…

Read More

Label Free Fluorescence made Easy, Request More Information

Connect with Avantes Today

  • Hidden
  • This field is for validation purposes and should be left unchanged.