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Detecting Amino Acid Dilution with Flow Cell Absorbance Spectroscopy

Posted on: June 27th, 2024

This experiment aims to track how the light absorption peaks of amino acids L-tyrosine and L-tryptophan change as their solutions are gradually diluted in deionized water. These amino acids, chosen for their unique light absorption properties in the ultraviolet range, were measured continuously using a flow cell and peristaltic pump. The pump mimics muscle contractions to move the solution through a tube, while the flow cell keeps the light source and detector at a fixed distance, a setup useful in various applications, including blood analysis.

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