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Absorbance Testing on Weed Killer Using Spectroscopy Instruments

Posted on: November 3rd, 2022

Roundup Experiment

In this experiment, we tested low concentrations of glyphosate with a special variable path length dip probe with a narrow tip to measure the chemical’s absorbance. We tested the dip probe with a path length of 10mm and 5mm to also measure the limit of detection for the lower concentrations. As path length is inversely correlated with detection limits in an absorbance measurement, we predicted that the 10mm path length would give more accurate and reliable results. We tested with the 5mm path length to see if the shorter path length still detected the glyphosate and if it provided similar results to the 10mm path length.

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